GFP-markiertes SELENBP1 bzw. SEMO-1

Scientific Research

Nutrigenomics
GFP-markiertes SELENBP1 bzw. SEMO-1
Image: Katrin Erler/Karl Köhnlein

Publications

Info board in the Dornburger Str.25

Info board in the Dornburger Str.25

Image: Josephine Priebs

Prof. Dr. Lars-Oliver Klotz

PD Dr. Holger Steinbrenner

Research focuses

FOXO/DAF-16

FOXO/DAF-16

Picture: aus Klotz et al., 2015

FOXO-transcription factors: key proteins of insulin-signalling

SELENBP1 as methanethiol oxidase (MTO) in the intestine

SELENBP1 as methanethiol oxidase (MTO) in the intestine

Picture: aus Philipp et al., 2021

Selenium-binding protein 1 (SELENBP1): Role in redox and sulfur metabolism as well as cell differentiation

 

C. elegans with SEMI-1::GFP expression

C. elegans with SEMI-1::GFP expression

Image: Verena Alexia Ohse

In-vivo-characterization of SELENBP1-orthologs (SEMO-1, SEMI-1) in Caenorhabditis elegans

Reactive oxygen species (ROS): signalling and toxic effects

Reactive oxygen species (ROS): signalling and toxic effects

Picture: aus Klotz und Steinbrenner, 2017

Oxidative stress: from toxic effects to signal transduction

Selenium, element of the Greek moon goddess

Selenium, element of the Greek moon goddess

Image: LO Klotz

Selenium and selenoproteins

Methods and models

Lipid accumulation of HepG2 cells / SEMO-1::GFP in C. elegans overlay

Lipid accumulation of HepG2 cells / SEMO-1::GFP in C. elegans overlay

Image: Katrin Erler/Verena Alexia Ohse
  • In-vitro-models (Caco-2, 3T3-L1, L6C11, HepG2) to investigate cell differentiation, resistance against oxidative stress, energy metabolism and intracellular lipid accumulation
  • In-vivo-model (C. elegans) to investigate life span, stress resistance, neuropathological changes and metabolism in wildtyp and genetic-modified nematodes
  • molecular biological methods with focus on biochemistry, sulfurous and seleniferous compounds (e.g. glutathione) and oxidative stress
  • Overexpression and purification of recombinant proteins in E. coli